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Sabtu, 10 Desember 2011

Pemeriksaan Kadar Vitamin C dengan titrasi Iodium

Praktek Kimia AMAMI
Analisa Vitamin C dengan titrasi Iodium

Tujuan : 
Untuk mengetahui kadar vitamin C dalam kemasan saset minuman

Metode : Iodometri

Prinsip Kerja : 
Vitamin C dititrasi dengan Iodium pada waktu bersamaan terjadi perubahan larutan Iodium menjadi tidak berwarna sehingga titik akhir titrasi dengan mudah dapat diketahuidengan kelebikan indikator amylum 1% (kelebihan larutan iodium sedikit saja akan menjadi biru dengan demikian titrasi dihentikan saat larutan berwarna biru muda safir)

Reagen : 
larutan yodium 0,01 N  
larutan amylum 1%

Cara Kerja :
  • Timbang 4gram sampel, masukkan kedalam erlenmeyer
  • Tambahkan aquadest sebanyak 20ml
  • Titrasi larutan tersebut dengan larutan yodium dengan indikator larutan amylum 1% sebanyak 2 tetes
  • Titasi harus di lakukan dengan secepatnya, mungkin sebab senyawa - senyawa lain seperti glutation dan sistein dapat di oksidasi oleh larutan yodium (Lakukan pengerjaan secara duplo)


Rumus Perhitungan :
Vol.titrasi x 0,88 x 100 mg  = .... Mg / Kg
         Berat Sampel

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