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Selasa, 27 Desember 2011

Pemeriksaan Cholesterol

Pemeriksaan Cholesterol
Merk       : BioSystems (REF 11505)
Metode    : CHOD-PAP
Prinsip     : Kadar cholesterol ditentukan menurut reaksi :
Cholesterol Ester   Chol esterase >   Cholesterol  +  fatty acid
Cholesterol  chol oxidase      Cholesten-4,1-3    +    H2O2
2 H2O2  +  phenol  +  amino-4-antipyrine           peroksidase  4H2O + quinoneimine
Cara kerja:
-          Dimasukkan ke dalam tabung

Blanko
Standar
Sampel
Serum
Standar
Reagen kerja
-
-
1000 µl
-
10 µl
1000 µl
10 µl
-
1000 µl

-          Dicampur dan diinkubasi selama 10 menit pada suhu ruang.
-          Diukur pada Fotometer Eppendorf  ECOM-F 6124 pada panjang gelombang 500 nm.

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