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Kamis, 29 Desember 2011

Pemeriksaan Alkali Phosphatase

Alkali Phosphatase
Merk     :  BioSystems (REF 11518)
Metode  :  Kinetik (Increase reaction)
Prinsip    :  Alkali phosphatase mengkatalisa dalam media alkali yang mentransfer 4-nitrophenilphosphat dan 2-amino-2-metil-1-propanol (AMP) menjadi 4-nitrophenol. Kenaikan   4-nitrofenol diukur secara fotometri pada panjang gelombang 405 nm yang sebanding dengan aktivitas  alkali phosphatase dalam sampel. 
Cara kerja  :
-          Dipipet ke dalam tabung :
Reagen kerja
Sampel
500 µl
10 µl

-          Dicampur dan langsung diukur pada fotometer dengan panjang   gelombang 405 nm.

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