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Kamis, 01 September 2011

THROMBIN TIME (TT)


THROMBIN TIME (TT)

Prinsip        :  Plasma ditambah larutan Thrombin akan terjadi bekuan fibrin dan dicatat waktu pembekuannya.
Tujuan        :  Untuk menguji faktor I dan faktor XIII
Alat dan Reagensia :
    1. Sentrifuge dan tabungnya.
    1. Pipet tetes
    2. Clinipette
    3. Tabung reaksi 10 x 75 mm
    4. Waterbath 37° C
    5. Stop watch
    6. Kaitan logam / Ose
    7. Natrium Citrate 3,8 %
    8. NaCl 0,9 %
Bahan Pemeriksaan : darah vena dengan anticoagulant Natrium Citrat 3,8% 1 : 9
Teknik Kerja         :
1.       0,2 ml plasma citrat dimasukkan kedalam tabung reaksi
2.       Kemudian tambahkan 0,1 ml larutan thrombin, pada saat penambahan stopwatch dijalankan.
3.       Dicatat saat terjadi bekuan awal dan dihentikan stopwatch saat terjadi bekuan sempurna.
4.       Dicatat waktu dan hasilnya
5.       Dilakukan juga uji ini terhadap plasma normal.
Nilai Normal      :    
- Permulaan                 :  5 – 10 detik
- Sempurna                  :  60 detik

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