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Rabu, 13 Juli 2011

Hematologi

Pemeriksaan Hematokrit

Tujuan :
untuk mengukur volume sel darah merah dalam darah

Prinsip kerja :
bila darah di centrifuge akan terjadi pemadatan dari sel - sel darah merah , ketebalan atau tinngi kolom sel darah di ukur dan di nyakan sebagai persentase terhadap seluruh darah

Alat :
centrifuge mikrohematokrit
tabung kapiler yang di dalamnya di lapisi dengan heparin
skala hematokrit
penggaris
lilin/malam/sabun

Bahan pemeriksaan :
darah vena yang telah di beri antikoagulan / EDTA

Cara kerja :

  • isi tabung kapiler dengan darah hingga mencapai 3/4 bagian dari tabung
  • tutp bagian ujung tabung tempat masuk darah dengan penutup khusus
  • letakkan tabung kapiler ke dalam sentrifus dengan ujung tertutup di sebelah luar
  • putar sentifus dengan kecepatan 1600 rpm selama 3 - 5 menit
  • setalah di angkat terlihat adanya 3 lapisan yakni sel darah merah , buffy cook, dan lapisan plasma
Nilai normal :
  • laki - laki : 42 - 50 %
  • wanita : 39 - 48 %
Perhitungan :

Hematokrit =          Panjang lapisan eritrosit           x 100%  = ....... g/dL  = .......%
                      Panjang lapisan eritrosit + Plasma     



DAFTAR PUSTAKA
catatan hematologi 2010

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