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Minggu, 15 Januari 2012

Pemeriksaan Anti HBs

Pemeriksaan Anti HBs
Metode   :  Rapid  Strip Test
Merk       :  AIM
Prinsip    :  Anti HBs Ag Rapid Strip Test adalah suatu cara penetapan kualitatif imunologi yang cepat berdasarkan prinsip Sandwich Imunokromatografi Recombinan HBs Ag murni yang melapisi membran filter akan mendeteksi anti HBs yang spesipik dengan sensitifitas yang tinggi terdiri dari dua bagian , bagian test (T) dan bagian kontrol (C). Garis test (T) yang berwarna ungu akan timbul dalam waktu 30 detik sampai 15 menit jika terdapat anti HBs di dalam serum. Garis kontrol (C) yang berwarna ungu selalu timbul dalam  proses pengetesan.

Alat dan bahan :
-          Tabung reaksi
-          Rapid Stip Anti HBs Ag

Bahan pemeriksaan : 
Serum.

Cara kerja :
  • Alat test dan spesimen harus mencapai suhu kamar sebelum pengetesan.
  • Dibuka sampul sebelum alat pereaksi dipakai, diberi tanda identitas pasien pada alat .
  • Dicelupkan strip Test kedalam serum (tidak boleh melebihi garis hitam pada Strip). Volume minimum sampel ± 1000 µl.
  •  Dibaca hasil pengetesan antara 15 – 20 menit. Tidak disarankan membaca hasil test sesudah 30 menit.

Interpretasi Hasil :
  • Negatif : hanya timbul satu garis kontrol (C).
  • Positif : timbul dua garis berwarna ungu masing – masing pada daerah kontrol (C) dan daerah Test (T).
  • Test yang gagal : bila garis kontrol (C) tidak muncul.

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